Question

What is the time duration required for completion of one PCR Cycle?

• A. 1 to 2 minutes
• B. 3 to 4 minutes
• C. 5 to 6 minutes
• D. 7 to 8 minutes

Hint:

One PCR cycle has denaturation, annealing and extension steps.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept
PCR amplifies DNA through repeated cycles of denaturation, annealing and extension.

Step 2: Time for each step
Denaturation about 1 min, annealing about 1 min, extension about 1 to 2 min.

Step 3: Total
One cycle takes about 3 to 4 minutes.

Final Answer:
One PCR cycle takes about 3 to 4 minutes. \[ \boxed{\text{(B) }\text{3 to 4 minutes}} \]