Question

What is the primary disadvantage of using spinner flasks for hybridoma culture?

• A. Low oxygen transfer rate
• B. Non-uniform pH
• C. High shear stress
• D. Inability to scale-up

Hint:

When considering culture systems for large-scale processes, always assess the scalability and the ability to transfer oxygen efficiently.

Answer 1

The Correct Option is C

Spinner flasks, while providing good mixing, have limitations in scale-up due to low oxygen transfer rates at larger volumes. The inability to scale-up makes them unsuitable for industrial applications compared to bioreactors.