Question

What is ELISA?

Hint:

ELISA is commonly used in HIV detection tests.

Answer 1

Step 1: Full form.
ELISA = Enzyme Linked Immunosorbent Assay.
Step 2: Definition.
It is a biochemical test used to detect the presence of antigens or antibodies in a sample using enzyme-linked reactions.
Step 3: Working principle.
- Antigen or antibody is fixed to a solid surface.
- A complementary antibody or antigen linked to an enzyme is added.
- Substrate is added → color change indicates positive reaction.
Step 4: Applications.
- Diagnosis of diseases like HIV, hepatitis, etc.
- Detection of hormones, allergens, and toxins.
Step 5: Conclusion.
ELISA is a highly sensitive diagnostic test for antigen-antibody interactions.