Question:
Various techniques are used to identify the variety of a crop. In which of the following crops, the 'seedling illumination test' is used for variety identification?
Various techniques are used to identify the variety of a crop. In which of the following crops, the 'seedling illumination test' is used for variety identification?
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The seedling illumination test under UV light is a rapid, non-destructive physiological method used in seed testing to identify and verify the varietal purity of Oat seeds based on tissue fluorescence.
Updated On: Aug 3, 2026
- Oat
- Wheat
- Sorghum
- Pearl millet
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Verified By Collegedunia
The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept:
Maintaining genetic and varietal purity is a fundamental goal of seed technology.
To verify the identity of a cultivar, seed analysts employ various rapid laboratory tests (chemical, physical, or physiological) that serve as diagnostic tools to distinguish between different varieties without needing to grow the plants to maturity.
Step 2: Detailed Explanation:
The "seedling illumination test" (often integrated with the fluorescence test) is a specific diagnostic test used in the following manner:
1. Biochemical Basis: The test exploits differences in the accumulation of fluorescent compounds (such as specific phenolic compounds or avenins) within the root or shoot tissues of certain varieties.
2. Application in Oats (Avena sativa): This test is classically applied to oats.
When oat seedlings or germinated seeds are illuminated under ultraviolet (UV) light, different varieties exhibit distinct fluorescence patterns.
Specifically, yellow oat varieties can be distinguished from white/red oat varieties because the roots of certain cultivars emit a characteristic bright fluorescence under UV light, whereas others remain non-fluorescent.
This allows seed testers to rapidly detect varietal mixtures in seed lots before distribution.
3. Other Crops: In wheat (B), phenol tests or NaOH tests are more commonly used for varietal identification.
For sorghum (C) and pearl millet (D), chemical tests like the potassium hydroxide (KOH) test or seed coat color reactions are typically preferred.
Therefore, the seedling illumination/fluorescence test is a standard diagnostic method for oats.
Step 3: Final Answer:
The seedling illumination test is used for variety identification in oats, corresponding to option (A).
Maintaining genetic and varietal purity is a fundamental goal of seed technology.
To verify the identity of a cultivar, seed analysts employ various rapid laboratory tests (chemical, physical, or physiological) that serve as diagnostic tools to distinguish between different varieties without needing to grow the plants to maturity.
Step 2: Detailed Explanation:
The "seedling illumination test" (often integrated with the fluorescence test) is a specific diagnostic test used in the following manner:
1. Biochemical Basis: The test exploits differences in the accumulation of fluorescent compounds (such as specific phenolic compounds or avenins) within the root or shoot tissues of certain varieties.
2. Application in Oats (Avena sativa): This test is classically applied to oats.
When oat seedlings or germinated seeds are illuminated under ultraviolet (UV) light, different varieties exhibit distinct fluorescence patterns.
Specifically, yellow oat varieties can be distinguished from white/red oat varieties because the roots of certain cultivars emit a characteristic bright fluorescence under UV light, whereas others remain non-fluorescent.
This allows seed testers to rapidly detect varietal mixtures in seed lots before distribution.
3. Other Crops: In wheat (B), phenol tests or NaOH tests are more commonly used for varietal identification.
For sorghum (C) and pearl millet (D), chemical tests like the potassium hydroxide (KOH) test or seed coat color reactions are typically preferred.
Therefore, the seedling illumination/fluorescence test is a standard diagnostic method for oats.
Step 3: Final Answer:
The seedling illumination test is used for variety identification in oats, corresponding to option (A).
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