Monozygotic (identical) twins develop from a single fertilized egg that splits into two embryos. Since they originate from the same zygote, they are always the same sex. Dizygotic (fraternal) twins develop from two separate eggs fertilized by two different sperm. Because they are from separate zygotes, they can be different sexes and have different genetic makeup (like any siblings). A boy and a girl twin pair can only be fraternal twins. In vitro fertilization doesn't determine whether twins are monozygotic or dizygotic. 75\% genetic similarity is not a standard measure for twins.