Question

To diagnose a syndromic illness like meningitis, which PCR technique is most appropriate?

• A. Multiplex PCR
• B. Uniplex PCR
• C. Attributed PCR
• D. Nested PCR

Hint:

Multiplex PCR is best when one syndrome can be caused by many organisms.

Answer 1

The Correct Option is A

Concept: Multiplex PCR allows simultaneous detection of multiple pathogens in a single reaction. It is useful for syndromic diagnosis where several organisms can cause similar symptoms.

Step 1: Understand syndromic illness.
Meningitis can be caused by many pathogens such as bacteria, viruses and fungi.

Step 2: Need for multiple target testing.
A test that can detect multiple organisms at once is useful.

Step 3: Choose PCR type.
Multiplex PCR detects multiple genetic targets simultaneously.