Question

The true statement(s) regarding the carbonic anhydrase enzyme is/are

• A. It is involved in peptide bond cleavage.
• B. Redox inactive Zn\(^{2+}\) ion is involved in the catalytic activity of this enzyme.
• C. Activated M-OH (M = metal ion) acts as the nucleophile in the enzyme.
• D. The metal ion is coordinated to the side chain of histidine residues.

Hint:

Carbonic anhydrase uses a zinc ion at its active site, and the activated hydroxyl group acts as the nucleophile in the reaction.

Answer 1

The Correct Option is B, C, D

Step 1: Understanding the enzyme’s function.
Carbonic anhydrase is an enzyme that catalyzes the conversion of carbon dioxide to bicarbonate. The enzyme contains a zinc ion (Zn\(^{2+}\)) at its active site. The metal ion is crucial for the enzyme's catalytic function. The nucleophile in the enzyme is an activated hydroxyl group (M-OH).
Step 2: Conclusion.
The correct statements are (B) and (C).