Question

The pH indicator used for animal cell culture media is

• A. CaCl$_2$
• B. Phenol red
• C. Fetal Bovine Serum
• D. Phenolphthalein

Hint:

• Phenol red → Cell culture pH indicator
• Color change helps monitor medium condition

Answer 1

The Correct Option is B

Concept: Animal cell culture media require monitoring of pH to ensure optimal growth conditions.
• pH indicators are added to visually detect changes in acidity/alkalinity

Step 1: Identifying the indicator.

• Phenol red is commonly used in cell culture media
• It changes color based on pH:
• Yellow → acidic
• Red → neutral
• Pink → alkaline

Step 2: Analyzing options.

• CaCl$_2$: Salt, not an indicator → Incorrect.
• Phenol red: pH indicator → Correct.
• Fetal Bovine Serum: Nutrient supplement → Incorrect.
• Phenolphthalein: Laboratory indicator, not used in culture media → Incorrect.

Step 3: Selecting the correct answer.
Thus, phenol red is used as a pH indicator in animal cell culture media.