Question

The enzyme phosphofructokinase and the co-factor Mg$^{2+$ are involved during which of the following reactions of glycolysis?

• A. Phosphorylation–II
• B. Cleavage
• C. Isomerisation
• D. Phosphorylation–I

Hint:

Phosphofructokinase is the rate-limiting and most regulated enzyme of glycolysis.

Answer 1

The Correct Option is A

Step 1: Understanding the role of phosphofructokinase (PFK).
Phosphofructokinase is a key regulatory enzyme of glycolysis. It catalyzes the conversion of fructose-6-phosphate into fructose-1,6-bisphosphate.
Step 2: Requirement of Mg$^{2+$. The enzyme phosphofructokinase requires Mg$^{2+}$ as a co-factor to facilitate the transfer of a phosphate group from ATP.
Step 3: Identifying the reaction type.
This reaction is the second phosphorylation step of glycolysis and is therefore termed Phosphorylation–II.
Step 4: Conclusion.
Phosphofructokinase and Mg$^{2+}$ are involved in Phosphorylation–II of glycolysis.