Question

The amplified DNA fragments can be separated by ___________.

• A. autoradiography
• B. gel electrophoresis
• C. x-ray crystallography
• D. ultra centrifugation

Hint:

DNA is negatively charged and moves through a gel under an electric field according to size.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept
DNA is negatively charged, so in an electric field it moves towards the anode.

Step 2: Gel electrophoresis
In an agarose gel, fragments are sieved by size, with smaller pieces travelling farther than larger ones.

Step 3: Visualising
After staining with ethidium bromide, the bands are seen under UV light and can be cut out (elution).

Final Answer:
Amplified DNA fragments are separated by gel electrophoresis. \[ \boxed{\text{(B) }\text{gel electrophoresis}} \]