Question

Select the correct order of steps of plant tissue culture:
A. Explant sterilization
B. Selection of media
C. Excision of regenerated shoots
D. Inoculation of explants
E. Transfer of plantlets to sterilised soil

• A. D, B, A, C, E
• B. B, C, D, A, E
• C. B, C, A, D, E
• D. B, A, D, C, E

Hint:

Plant Tissue Culture Workflow:
1. Prepare media (B) $\to$ 2. Surface sterilize tissue (A) $\to$ 3. Inoculate onto media (D) $\to$ 4. Excise shoots (C) $\to$ 5. Hardening in soil (E).

Answer 1

The Correct Option is D

Step 1: Understanding the Question:
The question requires arranging the basic procedural workflow of plant tissue culture and micropropagation in correct chronological sequence.

Step 2: Key Formula or Approach:
Plant tissue culture progresses sequentially from nutrient preparation to aseptic explant establishment, shoot/root organogenesis, shoot multiplication, and acclimatization: Media Selection (B) $\rightarrow{}$ Explant Surface Sterilization (A) \to Inoculation (D) $\rightarrow{}$ Shoot Excision (C) $\rightarrow{}$ Acclimatization/Soil Transfer (E)

Step 3: Detailed Explation:

• 1. Selection/Preparation of Media (B): Formulating and autoclaving appropriate culture media (e.g., Murashige and Skoog medium) containing essential macronutrients, micronutrients, carbon source, and plant growth regulators (auxins and cytokinins).

• 2. Explant Sterilization (A): Surface sterilizing plant tissue explants (leaf, stem, or node) using chemical disinfectants (such as sodium hypochlorite or mercuric chloride) under aseptic conditions.

• 3. Inoculation of Explants (D): Transferring the sterile explants onto the nutrient culture medium inside a lamir air flow cabinet.

• 4. Excision of Regenerated Shoots (C): Separating and excising regenerated microshoots for subculturing or rooting media treatment once organogenesis occurs.

• 5. Transfer to Sterilised Soil (E): Hardening off developed plantlets and acclimatizing them in sterilised soil/greenhouse before field transfer.

Step 4: Fil Answer:
The correct sequence of plant tissue culture steps is B, A, D, C, E.