Question:

Pepsin and Trypsin are protein digesting enzymes. Their optimum pH values are 2 and 9.5 respectively. What is the location of these enzymes in the digestive tract?

Show Hint

Pepsin works in acidic gastric juice, while trypsin works in the alkaline medium of the small intestine.
Updated On: Nov 12, 2026
  • Pepsin: Duodenum: Trypsin: Stomach.
  • Pepsin: Stomach : Trypsin : Duodenum.
  • Pepsin: Stomach : Trypsin: Stomach.
  • Pepsin: Duodenum : Trypsin : Duodenum.
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The Correct Option is B

Solution and Explanation

Step 1: Understanding the Concept
Enzymes work best at their optimum pH, which depends on where they are secreted.

Step 2: Pepsin
Pepsin is secreted by gastric glands and works in strongly acidic gastric juice at pH about 2. So it acts in the stomach.

Step 3: Trypsin
Trypsin comes from the pancreatic juice and is activated in the duodenum. It works in the alkaline medium there at pH about 9.5.

Step 4: Conclusion
So pepsin is in the stomach and trypsin is in the duodenum.

Final Answer:
Pepsin acts in the stomach and trypsin acts in the duodenum. \[ \boxed{\text{(B) }\text{Pepsin: Stomach : Trypsin : Duodenum.}} \]
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