Question

Match the technique with its application. 

• A. A-1, B-2, C-3, D-4
• B. A-4, B-3, C-2, D-1
• C. A-1, B-3, C-2, D-4
• D. A-2, B-1, C-4, D-3

Hint:

Use the mnemonic \textbf{SNOW DROP} to remember blotting targets:
\textbf{S}outhern = \textbf{D}NA
\textbf{N}orthern = \textbf{R}NA
\textbf{O} = \textbf{O} (placeholder)
\textbf{W}estern = \textbf{P}rotein

Answer 1

The Correct Option is A

Step 1: Understanding the Concept:
These are fundamental laboratory techniques used extensively in molecular biology and biotechnology.
Each technique is uniquely designed to identify, analyze, or quantify specific types of biomolecules.

Step 2: Detailed Explanation:
Let's connect each technique to its standard laboratory application.
- PCR (Polymerase Chain Reaction) (A): This is an in vitro technique used to rapidly synthesize millions of copies of a specific DNA segment.
Its primary application is DNA amplification.
Thus, A matches with 1.
- Western blot (B): This is an analytical technique used to detect specific proteins in a sample of tissue homogenate or extract.
It relies on the use of target-specific antibodies.
Thus, B matches with 2.
- Northern blot (C): This technique is used to detect specific RNA sequences in a sample.
It is commonly used to study gene expression by measuring mRNA levels.
Thus, C matches with 3.
- Southern blot (D): This is the original blotting method developed by Edwin Southern.
It is used to detect specific DNA sequences in a complex DNA sample.
Thus, D matches with 4.

Step 3: Final Answer:
Matching these gives the sequence: A-1, B-2, C-3, D-4.
This perfectly aligns with option (A).