Question

Match the object given in Column I with respective sterilizing agent given in Column II

Column (I)	Column (II)
(A) Nutrient medium	(I) 20% ethyl alcohol treatment
(B) Glass wares	(II) autoclaving
(C) Explant	(III) use of hot air oven

• A. A - II, B - I, C - III
• B. A - II, B - III, C - I
• C. A - I, B - II, C - III
• D. A - III, B - II, C - I

Hint:

Medium is autoclaved, glassware goes in a hot air oven and explants are treated with alcohol.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept
Everything used in tissue culture must be sterile to avoid contamination.

Step 2: Match the columns
(A) Nutrient medium is sterilised by autoclaving, so A matches II.
(B) Glassware is sterilised in a hot air oven, so B matches III.
(C) Explant is surface sterilised with alcohol treatment, so C matches I.

Step 3: Conclusion
The matching is A - II, B - III, C - I.

Final Answer:
The correct matching is A - II, B - III, C - I. \[ \boxed{\text{(B) }\text{A - II, B - III, C - I}} \]