Question

Match the LIST-I with LIST-II

• A. A-II, B-I, C-IV, D-III
• B. A-I, B-II, C-III, D-IV
• C. A-IV, B-III, C-II, D-I
• D. A-III, B-II, C-I, D-IV

Hint:

Cloning Capacity Hierarchy:
Plasmid ($\le 10\ \text{kb}$) $<$ Bacteriophage ($8-25\ \text{kb}$) $<$ Cosmid ($23-40\ \text{kb}$) $<$ BAC ($\sim 300\ \text{kb}$) $<$ YAC ($1000\ \text{kb}$).

Answer 1

The Correct Option is A

Step 1: Understanding the Question:
The question asks to match different cloning vectors in LIST-I with their characteristic D insert capacity ranges in LIST-II.

Step 2: Key Formula or Approach:
Cloning vectors carry foreign D inserts of varying sizes:
• Plasmid: Small insert capacity ($\le 10\ \text{kb}$).

• Bacteriophage ($\lambda$ phage): Moderate insert capacity ($8 - 25\ \text{kb}$).

• Cosmid: Hybrid plasmid-$cos$ vector capacity ($23 - 40\ \text{kb}$).

• BAC (Bacterial Artificial Chromosome): High capacity ($100 - 300\ \text{kb}$).

Step 3: Detailed Explation:

• A. BAC $\to$ II. 300 kb: Bacterial Artificial Chromosomes derived from the F-factor plasmid can stably carry large genomic inserts up to $100 - 300\ \text{kb}$.

• B. Plasmid $\to$ I. $\le$ 10 kb: Standard plasmid vectors (e.g., pBR322) carry small foreign D fragments typically up to $10\ \text{kb}$.

• C. Bacteriophage $\to$ IV. 8-25 kb: Lambda replacement vectors efficiently package D inserts in the range of $8 - 25\ \text{kb}$.

• D. Cosmid $\to$ III. 23-40 kb: Cosmid vectors contain lambda $\cos$ sites and can be packaged $in\ vitro$ with inserts ranging from $23 - 40\ \text{kb}$.

Step 4: Fil Answer:
The correct matching sequence is A-II, B-I, C-IV, D-III.