Question

Match the Column-I with Column-II and select the correct option.
Column-I Column-II
(a) DNA fragmentation (i) ssDNA gets embedded in nylon membrane
(b) Electrophoresis (ii) Sample DNA and probe DNA form double stranded DNA
(c) Southern blotting (iii) DNA fragments move to positive pole
(d) Hybridization (iv) Fragments of DNA with variable length are formed

• A. a–(iv), b–(ii), c–(i), d–(iii)
• B. a–(iii), b–(ii), c–(iv), d–(i)
• C. a–(i), b–(ii), c–(iii), d–(iv)
• D. a–(iv), b–(iii), c–(i), d–(ii)

Hint:

Remember: Fragmentation → variable lengths, Electrophoresis → movement to +ve pole, Southern blot → membrane transfer, Hybridization → probe binding.

Answer 1

The Correct Option is D

Step 1: Understanding DNA fragmentation.
DNA fragmentation involves cutting DNA using restriction enzymes, producing fragments of variable lengths depending on recognition sites.
Step 2: Electrophoresis principle.
DNA molecules are negatively charged and move towards the positive pole during gel electrophoresis under an electric field.
Step 3: Southern blotting process.
In Southern blotting, single-stranded DNA fragments are transferred and embedded onto a nylon or nitrocellulose membrane.
Step 4: Hybridization step.
Hybridization involves the binding of complementary DNA strands where the probe DNA forms double-stranded DNA with the target sequence.
Step 5: Conclusion.
The correct matching is option (D).