Question

Match the Column I containing restriction enzymes with Column II containing recognition sequences.

• A. i - a ii - d iii - c iv - b
• B. i - b ii - c iii - d iv - a
• C. i - c ii - b iii - d iv - a
• D. i - a ii - c iii - d iv - b

Hint:

- EcoRI = sticky ends (GAATTC) - BamHI = GGATCC - AluI = blunt cutter (AGCT) - HindII = blunt cutter

Answer 1

The Correct Option is A

Step 1: Identify sequences

• AluI $\rightarrow$ AGCT (blunt cut)
• BamHI $\rightarrow$ GGATCC
• EcoRI $\rightarrow$ GAATTC
• HindII $\rightarrow$ GTAC type (blunt cutter)

Step 2: Match
\[ i-a,\quad ii-d,\quad iii-c,\quad iv-b \]