Question:

Match List-I with List-II

List-I (n for Infant milk food)List-II (Organism)
(A) 60(I) Cronobacter sakazakii
(B) 30(II) Salmonella spp
(C) 10(III) Bacillus cereus
(D) 5(IV) Enterobacteriaceae

Choose the correct answer from the options given below

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To remember the sampling plans for infant milk foods:
- Highly critical pathogens require large sample sizes: Salmonella (\(n = 60\)) and Cronobacter (\(n = 30\)).
- Process hygiene indicators require moderate sample sizes: Enterobacteriaceae (\(n = 10\)).
- Sporeformers with partial tolerance require small sample sizes: Bacillus cereus (\(n = 5\)).
  • (A) - (I), (B) - (II), (C) - (III), (D) - (IV)
  • (A) - (I), (B) - (III), (C) - (IV), (D) - (II)
  • (A) - (II), (B) - (I), (C) - (IV), (D) - (III)
  • (A) - (I), (B) - (IV), (C) - (III), (D) - (II)
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The Correct Option is C

Solution and Explanation

Step 1: Understanding the Concept:
Microbiological standards for infant milk foods use a three-class sampling plan to protect vulnerable infants from pathogens.
In these plans, \(n\) represents the number of sample units that must be collected and analyzed from a single lot.
A larger value of \(n\) increases the sensitivity of the sampling plan, making it highly suitable for screening critical pathogens.

Step 2: Detailed Explanation:

Let us analyze the FSSAI and Codex microbiological criteria for infant milk foods:
- (A) n = 60 matches with (II) Salmonella spp.:
Because Salmonella is a highly virulent pathogen with zero tolerance in infant formulas, a very large sample size of \(n = 60\) is required to detect even extremely low levels of contamination.
The criteria specify that Salmonella must be absent in all 60 samples of 25 g each.
- (B) n = 30 matches with (I) Cronobacter sakazakii:
Cronobacter sakazakii (formerly Enterobacter sakazakii) is an opportunistic pathogen that can cause neonatal meningitis and necrotizing enterocolitis.
The sampling standard requires \(n = 30\) sample units of 10 g each, with zero allowable positives (\(c = 0\)).
- (C) n = 10 matches with (IV) Enterobacteriaceae:
Enterobacteriaceae serves as a process hygiene indicator to monitor sanitization and post-processing contamination.
Because it is an indicator group rather than a primary pathogen, a sample size of \(n = 10\) is used.
- (D) n = 5 matches with (III) Bacillus cereus:
Bacillus cereus is a spore-forming bacterium allowed in low numbers in infant formulas.
The sampling plan sets \(n = 5\) with \(c = 1\) (one sample can contain between 50 and 100 CFU/g).
This matching sequence corresponds to Option (C).

Step 3: Final Answer:

The correct matching sequence is (A) - (II), (B) - (I), (C) - (IV), (D) - (III).
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