Question

Match List-I with List-II
{|p{7cm}|p{7cm}|} List-I & List-II
(A) Southern hybridization & (I) Western blotting
(B) Presence of protein of interest & (II) Y-2H
(C) Peptide sequence & (III) Integration of transgene
(D) Interaction of two transcription factors & (IV) Mass spectrometry

• (A) - (V), (B) - (IV), (C) - (III), (D) - (I)
• (A) - (IV), (B) - (V), (C) - (II), (D) - (III)
• (A) - (I), (B) - (II), (C) - (III), (D) - (IV)
• (A) - (III), (B) - (I), (C) - (IV), (D) - (II)

Hint:

Remember the molecular biology blotting directions:
- Southern Blot $\rightarrow$ DNA detection.
- Northern Blot $\rightarrow$ RNA detection.
- Western Blot $\rightarrow$ Protein detection.

Answer 1

The Correct Option is D

Step 1: Understanding the Concept:
Molecular biotechnology relies on specific assays to analyze nucleic acids, proteins, and their physical interactions.
Matching these analytical techniques with their corresponding experimental applications is essential for molecular diagnostics and research.

Step 2: Detailed Explanation:
Let us evaluate each of the listed methods and their biological applications:
(A) Southern hybridization:
This technique is used to detect specific DNA sequences in genomic samples.
In plant genetic engineering, it is the standard method used to confirm the stable integration and determine the copy number of a transgene in the host genome.
Hence, (A) matches with (III).
(B) Presence of protein of interest:
Western blotting (immunoblotting) uses specific antibodies to detect and quantify target proteins that have been separated by gel electrophoresis.
Hence, (B) matches with (I).
(C) Peptide sequence:
Mass spectrometry (MS) measures the mass-to-charge ratio of gas-phase ions generated from peptides.
In tandem MS/MS, peptides are fragmented to determine their amino acid sequence directly.
Hence, (C) matches with (IV).
(D) Interaction of two transcription factors:
The Yeast Two-Hybrid (Y-2H) system is a powerful in vivo genetic assay used to screen for and confirm physical interactions between two target proteins, such as transcription factors.
Hence, (D) matches with (II).

Step 3: Final Answer:
The correct matching sequence is:
(A) - (III), (B) - (I), (C) - (IV), (D) - (II).
This corresponds to Option (D).