Question

Match List-I with List-II


List-I (Name of the Enzyme) & List-II (Class of the enzyme)
(A). Pyruvate carboxylase & (I). Oxidoreductase
(B). Fumarase & (II). Hydrolase
(C). Phospholipase & (III). Lyase
(D). Nitrogenase & (IV). Ligase


Choose the correct answer from the options given below:

• (A) - (I), (B) - (II), (C) - (III), (D) - (IV)
• (A) - (IV), (B) - (III), (C) - (II), (D) - (I)
• (A) - (I), (B) - (III), (C) - (IV), (D) - (II)
• (A) - (IV), (B) - (I), (C) - (III), (D) - (II)

Hint:

Remember that all carboxylases that require ATP to join molecules are classified under Ligases (Class 6), while those that do not utilize ATP are typically Lyases.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
Enzymes are systematically classified into six major classes by the Enzyme Commission (EC) based on the specific type of chemical reaction they catalyze.

Step 2: Detailed Explanation:
Let us match each enzyme with its correct EC class:
- (A) Pyruvate carboxylase catalyzes the covalent joining of carbon dioxide to pyruvate to form oxaloacetate, utilizing energy from ATP hydrolysis. This makes it a Ligase (IV).
- (B) Fumarase catalyzes the reversible non-hydrolytic addition of water to the double bond of fumarate, forming malate. This makes it a Lyase (III).
- (C) Phospholipase hydrolyzes ester bonds within phospholipids using water, classifying it as a Hydrolase (II).
- (D) Nitrogenase catalyzes the chemical reduction of dinitrogen gas (\(\text{N}_2\)) to ammonia (\(\text{NH}_3\)) by transferring electrons, classifying it as an Oxidoreductase (I).
Combining these gives the correct matching sequence: (A)-(IV), (B)-(III), (C)-(II), (D)-(I).

Step 3: Final Answer:
Hence, the correct option is (B).