Question

Match List-I with List-II
{|p{7cm}|p{7cm}|} List-I (Book/Theory proposed/Characteristic, etc.) & List-II (Author/Thinker/Name of Theory, etc.)
(A) Schiffs Reagent & (I) Nucleic Acid to Protein
(B) Central Dogma & (II) DNA
(C) Chromosome & (III) Feulgen reaction
(D) Ultraviolet light of 2600 angstroms & (IV) DNA and Histone proteins

• (A) - (IV), (B) - (II), (C) - (I), (D) - (III)
• (A) - (III), (B) - (IV), (C) - (I), (D) - (II)
• (A) - (I), (B) - (III), (C) - (IV), (D) - (II)
• (A) - (III), (B) - (I), (C) - (IV), (D) - (II)

Hint:

The absorption of UV light by nucleic acids at $260\text{ nm}$ ($2600\text{ \AA}$) is a key biophysical property used in spectrophotometry to measure DNA/RNA concentration and assess purity by calculating the $A_{260}/A_{280}$ ratio.

Answer 1

The Correct Option is D

Step 1: Understanding the Concept:
This matching question covers fundamental biochemical reagents, genetic theories, chromosome structures, and the physical properties of nucleic acids.

Step 2: Detailed Explanation:
Let us analyze each pair individually to determine the correct matches:
(A) Schiffs Reagent:
Schiff's reagent is a chemical used in the Feulgen staining reaction, which is a cytochemical method developed by Robert Feulgen to stain chromosomal DNA.
Acid hydrolysis of DNA exposes aldehyde groups, which then react with Schiff's reagent to produce a deep red-purple color.
Hence, (A) matches with (III).
(B) Central Dogma:
Proposed by Francis Crick, the Central Dogma describes the directional flow of genetic information inside biological systems from DNA to RNA, and finally from nucleic acid to functional proteins.
Hence, (B) matches with (I).
(C) Chromosome:
Eukaryotic chromosomes consist of chromatin, which is composed of genomic DNA wrapped around octamers of basic histone proteins to form nucleosomes.
Hence, (C) matches with (IV).
(D) Ultraviolet light of 2600 Angstroms ($260\text{ nm}$):
Purine and pyrimidine bases in DNA absorb ultraviolet light strongly, with a maximum absorption peak at $260\text{ nm}$ ($2600\text{ \AA}$).
This property is used to quantify DNA concentration in solutions.
Hence, (D) matches with (II).

Step 3: Final Answer:
The correct matching sequence is:
(A) - (III), (B) - (I), (C) - (IV), (D) - (II).
This corresponds to Option (D).