Question:
Match List-I with List-II
List-I (Stain) List-II (Used to identify) (A) Janus Green B (I) Glycogen (B) Periodic Acid Schiff (II) Collagen (C) Sudan III stain (III) Mitochondria (D) Masson's Trichome (IV) Lipids
Match List-I with List-II
| List-I (Stain) | List-II (Used to identify) |
|---|---|
| (A) Janus Green B | (I) Glycogen |
| (B) Periodic Acid Schiff | (II) Collagen |
| (C) Sudan III stain | (III) Mitochondria |
| (D) Masson's Trichome | (IV) Lipids |
Show Hint
- Janus Green B = Mitochondria.
- PAS = Glycogen.
- Sudan III Oil Red O = Lipids.
- Masson's Trichrome = Collagen.
- PAS = Glycogen.
- Sudan III Oil Red O = Lipids.
- Masson's Trichrome = Collagen.
Updated On: Jul 23, 2026
- (A) - (IV), (B) - (II), (C) - (III), (D) - (I)
- (A) - (II), (B) - (I), (C) - (III), (D) - (IV)
- (A) - (III), (B) - (I), (C) - (II), (D) - (IV)
- (A) - (III), (B) - (I), (C) - (IV), (D) - (II)
Show Solution
Verified By Collegedunia
The Correct Option is D
Solution and Explanation
Step 1: Understanding the Concept:
Histological staining techniques utilize specific chemical affinities between dye molecules and intracellular or extracellular components to selectively visualize tissues under a microscope.
Step 2: Detailed Explanation:
Let us match the stains in List I with their target structures in List II:
- (A) Janus Green B:
Janus Green B is a vital stain that selectively accumulates inside living mitochondria due to the active cytochrome oxidase enzyme system present in these organelles, which keeps the dye in its oxidized, colored state.
Thus, (A) matches with (III).
- (B) Periodic Acid Schiff (PAS):
PAS is a classic histochemical reaction used to detect polysaccharides, particularly glycogen and mucopolysaccharides, by oxidizing adjacent glycol groups to aldehydes, which then react with Schiff's reagent to produce a magenta color.
Thus, (B) matches with (I).
- (C) Sudan III stain:
Sudan III is a lysochrome (fat-soluble) dye used for the histochemical demonstration of neutral lipids, triglycerides, and fats in frozen tissue sections, staining them a bright red-orange color.
Thus, (C) matches with (IV).
- (D) Masson's Trichrome:
Masson's Trichrome is a multi-step staining protocol widely used to differentiate collagen fibers from muscle tissue, staining collagen fibers bright blue or green, cytoplasm/muscle red, and nuclei dark brown/black.
Thus, (D) matches with (II).
Combining these matches, we get: (A)-(III), (B)-(I), (C)-(IV), (D)-(II).
Step 3: Final Answer:
The correct option is (D).
Histological staining techniques utilize specific chemical affinities between dye molecules and intracellular or extracellular components to selectively visualize tissues under a microscope.
Step 2: Detailed Explanation:
Let us match the stains in List I with their target structures in List II:
- (A) Janus Green B:
Janus Green B is a vital stain that selectively accumulates inside living mitochondria due to the active cytochrome oxidase enzyme system present in these organelles, which keeps the dye in its oxidized, colored state.
Thus, (A) matches with (III).
- (B) Periodic Acid Schiff (PAS):
PAS is a classic histochemical reaction used to detect polysaccharides, particularly glycogen and mucopolysaccharides, by oxidizing adjacent glycol groups to aldehydes, which then react with Schiff's reagent to produce a magenta color.
Thus, (B) matches with (I).
- (C) Sudan III stain:
Sudan III is a lysochrome (fat-soluble) dye used for the histochemical demonstration of neutral lipids, triglycerides, and fats in frozen tissue sections, staining them a bright red-orange color.
Thus, (C) matches with (IV).
- (D) Masson's Trichrome:
Masson's Trichrome is a multi-step staining protocol widely used to differentiate collagen fibers from muscle tissue, staining collagen fibers bright blue or green, cytoplasm/muscle red, and nuclei dark brown/black.
Thus, (D) matches with (II).
Combining these matches, we get: (A)-(III), (B)-(I), (C)-(IV), (D)-(II).
Step 3: Final Answer:
The correct option is (D).
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