Question

Match List - I with List - II.

List - I	List - II
A. SDS-PAGE	I. DNA
B. Agarose gel electrophoresis	II. Liquid chromatography
C. Native PAGE	III. Non-denatured proteins
D. HPLC	IV. Proteins

Choose the correct answer from the options given below:

• A. A-II, B-IV, C-III, D-I \
• B. A-IV, B-I, C-III, D-II \
• C. A-I, B-II, C-III, D-IV \
• D. A-III, B-II, C-IV, D-I

Hint:

"Native" implies natural and undamaged, perfectly matching non-denatured proteins. Agarose is the absolute standard matrix for running DNA gels.

Answer 1

The Correct Option is B

Step 1: Concept Analytical separation methods distinguish macromolecules or chemical species using differences in charge, size, hydrophobicity, or molecular mass.

Step 2: Meaning * SDS-PAGE: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (denatures proteins). * Native PAGE: Polyacrylamide Gel Electrophoresis run without denaturing detergents. * HPLC: High-Performance Liquid Chromatography.

Step 3: Analysis * SDS-PAGE (A) uses anionic detergent to unfold and coat proteins with negative charges, separating total protein subunits primarily by mass $\rightarrow$ (A-IV). * Agarose gel electrophoresis (B) utilizes a large-pore matrix perfect for resolving larger polyanionic DNA polymers $\rightarrow$ (B-I). * Native PAGE (C) keeps proteins in their fully native, active folded three-dimensional conformations during the run $\rightarrow$ (C-III). * HPLC (D) utilizes high pressure to pump liquid solvents containing analyte mixtures through specialized separation columns $\rightarrow$ (D-II).

Step 4: Conclusion The resulting unified map is A-IV, B-I, C-III, D-II, which corresponds to option B. Final Answer: (B)