Question

Match List I with List II
List I (Vector):
A. pBR322
B. pUC8
C. pGEM3Z
D. M13mp1

List II (Description):
I. Vector facilitating in vitro transcription
II. Cloning vector based on bacteriophage
III. E. coli plasmid vector to clone DNA
IV. A lac selection plasmid
V. F plasmid based vector for handling longer DNA inserts for genomic library construction

Choose the correct answer from the options given below:

• A. A - III, B - IV, C - I, D - II
• B. A - I, B - III, C - II, D - IV
• C. A - II, B - I, C - III, D - IV
• D. A - IV, B - II, C - III, D - V

Hint:

Vector references:
pBR322 $\rightarrow$ Classic E. coli cloning plasmid.
pUC series $\rightarrow$ lacZ alpha-complementation (blue-white selection).
pGEM series $\rightarrow$ T7/SP6 promoters for in vitro transcription.
M13 series $\rightarrow$ Single-stranded bacteriophage vector.

Answer 1

The Correct Option is A

Step 1: Understanding the Concept:
Cloning vectors are specialized DNA molecules used to carry and replicate foreign DNA segments in host cells.
Different vectors are engineered with specific features, such as selection markers, promoters, or phage packaging systems, depending on the application.

Step 2: Detailed Explanation:
Let us match the vectors in List I with their corresponding descriptions in List II:
1. pBR322 (A): One of the first widely used, standard synthetic plasmid vectors engineered for E. coli cloning.
It contains genes for ampicillin and tetracycline resistance as selectable markers. Thus, A matches with III.
2. pUC8 (B): A small plasmid vector developed at the University of California.
It features a multiple cloning site (MCS) located within the $\textit{lacZ'}$ gene, allowing for blue-white colony screening (lac selection) through $\alpha$-complementation. Thus, B matches with IV.
3. pGEM3Z (C): A transcription plasmid vector designed for in vitro transcription.
It contains bacteriophage T7 and SP6 RNA polymerase promoters flanking the MCS, allowing for the synthesis of single-stranded RNA probes from cloned DNA. Thus, C matches with I.
4. M13mp1 (D): A cloning vector derived from the filamentous E. coli bacteriophage M13.
It is used to obtain single-stranded DNA templates for sequencing and mutagenesis. Thus, D matches with II.
This matches the sequence: A - III, B - IV, C - I, D - II.

Step 3: Final Answer:
The correct matching sequence is A - III, B - IV, C - I, D - II, which corresponds to Option (A).