Question

Match List I with List II
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Choose the correct answer from the options given below:}

• A. A-I, B-V, C-II, D-IV
• B. A-V, B-IV, C-I, D-II
• C. A-II, B-I, C-IV, D-III
• D. A-II, B-III, C-IV, D-V

Hint:

Mass Spec = Protein ID. BiFC = Live cell interaction. Homologous Recombination = Knockout. Functional Genomics = Studying the "Action" of the genome.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept:
Biotechnology involves various specialized methods to analyze gene function, protein structure, and molecular interactions. Functional genomics seeks to assign functions to genomic data.

Step 2: Detailed Explanation:
- A. Functional genomics: A major part of determining what a gene does involves studying its protein product's interactions. The study of protein interactions is a hallmark of functional genomics. (Match: A-II).
- B. Gene inactivation: This is often achieved through "gene knockouts." In many organisms, specifically yeast and mice, gene knockouts are performed via Homologous Recombination, where a defective copy of the gene replaces the functional one. (Match: B-I).
- C. In-vivo protein-protein interaction: Bimolecular Fluorescence Complementation (BiFC) is a powerful tool to visualize protein interactions directly inside living cells by splitting a fluorescent protein (like GFP) and attaching the halves to interacting partners. (Match: C-IV).
- D. Identification of unknown protein: The definitive technique for identifying unknown proteins in a sample (Proteomics) is Mass spectrometry, which determines the mass-to-charge ratio of peptide fragments. (Match: D-III).
Note: Yeast 2-hybrid (V) is also an interaction tool, but based on the specific structure of the lists provided in the paper, the pairing A-II, B-I, C-IV, D-III provides the most accurate methodological classification.

Step 3: Final Answer:
Matching the biological fields/goals with their techniques results in A-II, B-I, C-IV, D-III. Option 3 is correct.