Question

Match List - I with List - II. 

Choose the correct answer from the options given below : 
 

• A. A-IV, B-I, C-II, D-III
• B. A-I, B-III, C-IV, D-II
• C. A-IV, B-III, C-I, D-II
• D. A-II, B-I, C-III, D-IV

Hint:

Microbiology matching questions often hinge on one highly specific clinical fact. If you know that Bacteroides requires Vitamin K and Hemin (a standard lab cultivation rule), you can immediately narrow a 4-option question down to a 50/50 guess.

Answer 1

The Correct Option is C

Step 1: Concept:
Certain bacterial species are fastidious, meaning they require specific essential nutrients or growth factors (like vitamins) in their culture media because they lack the metabolic pathways to synthesize them.

Step 2: Step-by-step Explanation:

• B. Vitamin K: Bacteroides melaninogenicus (now often reclassified as Prevotella melaninogenica) is a famous strict anaerobe that requires hemin and Vitamin K (menadione) as essential growth factors to synthesize elements of its electron transport chain. (B $\rightarrow$ III). This strongly points to options 2 or 3.

• C. Thiamine ($B_1$): Bacillus anthracis requires the addition of specific vitamins, principally thiamine, to support robust growth in defined minimal media. (C $\rightarrow$ I). This isolates option 3 as the only possibility.

• A. Riboflavin: Clostridium tetani has a known requirement for specific B vitamins including riboflavin to thrive. (A $\rightarrow$ IV).

• D. Folic Acid: Leuconostoc species (lactic acid bacteria) are known to be highly fastidious and require complex mixtures of vitamins, with Folic acid being a classical required growth factor for L. dextranicum. (D $\rightarrow$ II).
The full matched sequence is A-IV, B-III, C-I, D-II.

Step 3: Final Answer:
The matching pairs correspond to option (C).