Question

Match List-I with List-II:

Choose the correct answer from the options given below:

• (A) - (I), (B) - (V), (C) - (II), (D) - (IV)
• (A) - (V), (B) - (IV), (C) - (I), (D) - (III)
• (A) - (III), (B) - (I), (C) - (IV), (D) - (II)
• (A) - (II), (B) - (III), (C) - (IV), (D) - (V)

Hint:

Remember: RT-PCR is always linked to RNA/cDNA amplification, and CaCl\(_2\) treatment is the standard chemical method for generating competent bacterial cells.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept:
This matching exercise links fundamental genetic engineering methods (DNA amplification, bacterial transformation, clone verification) with their corresponding molecular tools.

Step 2: Detailed Explanation:
Let us analyze each pair:
- Amplification of a known DNA sequence: Polymerase Chain Reaction (PCR) is used to amplify target DNA sequences using sequence-specific primers and a thermostable DNA polymerase.
Therefore, (A) matches with (III).
- Amplification of cDNA sequence: Reverse Transcription PCR (RT-PCR) reverse-transcribes RNA into complementary DNA (cDNA), which is then amplified by PCR.
Therefore, (B) matches with (I).
- Selection of transformed cells: Colony PCR is used to quickly determine if transformed bacterial colonies contain the target insert without requiring plasmid purification.
Therefore, (C) matches with (IV).
- CaCl\(_2\) (Calcium chloride): Treatment of bacterial cells with calcium chloride, followed by heat shock, neutralizes negative charges on DNA and the cell membrane, rendering the cells competent to take up foreign DNA.
Therefore, (D) matches with (II).
This pairing gives: (A) - (III), (B) - (I), (C) - (IV), (D) - (II).

Step 3: Final Answer:
Hence, the correct option is (C).