Question

Match List-I with List-II:

Choose the correct answer from the options given below:

• (A) - (V), (B) - (III), (C) - (I), (D) - (IV)
• (A) - (IV), (B) - (V), (C) - (I), (D) - (II)
• (A) - (II), (B) - (IV), (C) - (III), (D) - (I)
• (A) - (I), (B) - (V), (C) - (III), (D) - (II)

Hint:

For rapid matching, link the poly-A tail of mature mRNA to oligo-dT columns, and match plasmid isolation to alkaline lysis.

Answer 1

The Correct Option is A

Step 1: Understanding the Concept:
Molecular biology techniques rely on specific physical, chemical, and structural properties of macromolecules (plasmid, mRNA, proteins, genomic sequences) to isolate or separate them.

Step 2: Detailed Explanation:
Let us analyze each pair:
- Isolation of CDS (Coding Sequence): Eukaryotic coding sequences contain only exons, with all non-coding introns spliced out.
A cDNA library is prepared by reverse-transcribing mature processed mRNA, meaning it represents only the coding sequences (CDS).
Therefore, (A) matches with (V).
- Isolation of plasmid: Plasmid DNA is isolated using the alkaline lysis method.
Sodium hydroxide denatures both genomic and plasmid DNA, but selective neutralization allows small plasmids to rapidly reanneal while genomic DNA precipitates out.
Therefore, (B) matches with (III).
- Isolation of mRNA: Mature eukaryotic mRNA molecules possess a poly-A tail at their \(3'\) end.
This poly-A tail hybridizes selectively with an oligo-dT column in affinity chromatography, allowing mRNA purification.
Therefore, (C) matches with (I).
- Separation of proteins: Gel electrophoresis, specifically SDS-PAGE, separates proteins based on molecular weight by applying an electric current through a polyacrylamide matrix.
Therefore, (D) matches with (IV).
Combining these associations yields: (A) - (V), (B) - (III), (C) - (I), (D) - (IV).

Step 3: Final Answer:
Hence, the correct option is (A).