Question

Match List - I with List - II.

Choose the correct answer from the options given below:

• A. A-I, B-II, C-IV, D-III
• B. A-II, B-IV, C-III, D-I
• C. A-IV, B-III, C-II, D-I
• D. A-IV, B-II, C-I, D-III

Hint:

Remember: Pectinase = "Glue-dissolver" (Middle lamella). Phenosafranin = "Death-marker" (Stains dead cells).

Answer 1

The Correct Option is C

Concept: This matching question covers various chemical agents used in plant biotechnology, ranging from enzymes for cell isolation to dyes for viability testing and herbicides for selection.

Step 1: Enzymes and Sugars.
Pectinase (A) is the enzyme used to dissolve pectin, which is the primary component of the middle lamella (IV) that holds plant cells together. Mannitol (B) is a sugar alcohol commonly used as an osmotic stabilizer because it is a metabolically inactive sugar (III), meaning the plant cells do not consume it, allowing for stable osmotic pressure.

Step 2: Stains and Herbicides.
Phenosafranin (C) is a dye used in cell culture to stain dead cells (II) red, while living cells with intact membranes exclude the dye. This is a common method for assessing culture viability. Atrazine (D) is a widely known herbicide (I) that inhibits photosynthesis; in biotechnology, it is often used as a selective agent for resistant cell lines.

Step 3: Conclusion.
Matching these pairs results in the sequence: A-IV, B-III, C-II, D-I.