Question

Match LIST-I with LIST-II 

LIST-I		LIST-II
A.	Sea weeds	I.	Isolation of DNA from gel
B.	Staining of DNA	II.	Gel electrophoresis
C.	Elution	III.	Source of agarose
D.	Separation of DNA fragments	IV.	Ethidium bromide

Choose the correct answer from the options given below: 
 

• A. A-III, B-IV, C-II, D-I
• B. A-III, B-IV, C-I, D-II
• C. A-II, B-IV, C-I, D-III
• D. A-III, B-II, C-IV, D-I

Hint:

Visualize the entire gel electrophoresis process to answer these questions: You make the gel with agarose from {sea weeds} (A-III). You run the gel for {separation} (D-II). You {stain} with EtBr to see the DNA (B-IV). You cut out the band and {elute} it to get the pure DNA (C-I).

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
This question requires matching various components and steps related to the technique of agarose gel electrophoresis with their definitions or associated items.
Step 2: Detailed Explanation:
A. Sea weeds: Agarose, the polysaccharide that forms the gel matrix for electrophoresis, is extracted from certain types of red III. sea weeds (e.g., from the genera Gelidium and Gracilaria).
B. Staining of DNA: To visualize the DNA fragments in the gel after electrophoresis, a fluorescent dye is used. The most classic and common staining agent is IV. Ethidium bromide, which intercalates between the DNA base pairs and fluoresces under UV light.
C. Elution: This is the process of extracting and purifying a specific DNA band from the agarose gel after it has been separated and visualized. It is the I. Isolation of DNA from gel.
D. Separation of DNA fragments: The core purpose of II. Gel electrophoresis is to separate macromolecules like DNA, RNA, or proteins. In the case of DNA, it separates fragments based on their size.
Step 3: Final Answer:
The correct matches are A-III, B-IV, C-I, D-II.