Question

Match LIST-I with LIST-II 

LIST-I		LIST-II
A.	Gene inhibition	I.	Addition of functional gene to their genome to replace missing product
B.	Gene editing	II.	Disarm the product of faulty gene
C.	Gene targeting	III.	CRISPR/Cas9
D.	Gene Augmentation therapy	IV.	Replacement of non-functional gene with normal gene

Choose the correct answer from the options given below: 
 

• A. A-III, B-IV, C-II, D-I
• B. A-II, B-III, C-IV, D-I
• C. A-I, B-II, C-III, D-IV
• D. A-IV, B-III, C-I, D-II

Hint:

Remember the core ideas: {Augmentation} = Adding a good copy. {Inhibition} = Stopping a bad copy. {Editing} = Making precise changes (think CRISPR). {Targeting/Replacement} = Swapping out a gene.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
This question requires matching different gene therapy strategies (LIST-I) with their corresponding descriptions or mechanisms (LIST-II).
Step 2: Detailed Explanation:
A. Gene inhibition: This strategy is used for diseases caused by a faulty gene that produces a harmful product (gain-of-function mutation). The goal is to stop or 'inhibit' the gene from working. This can be done by blocking transcription or translation. This matches with II. Disarm the product of faulty gene.
B. Gene editing: This refers to the technology that allows scientists to make precise changes to the DNA sequence of an organism. The most well-known and versatile tool for this is III. CRISPR/Cas9.
C. Gene targeting: This is a specific technique that uses homologous recombination to modify an endogenous gene. It can be used to create specific mutations, correct a faulty gene, or replace a gene. This matches well with IV. Replacement of non functional gene with normal gene.
D. Gene Augmentation therapy: This is the most common form of gene therapy, used for diseases caused by the loss of a gene's function (e.g., cystic fibrosis). The strategy is to introduce a healthy, functional copy of the gene into the cells to compensate for the faulty one. This matches perfectly with I. Addition of functional gene to their genome to replace missing product.
Step 3: Final Answer:
The correct matching is: A-II, B-III, C-IV, D-I.