Question

Marker enzyme of lysosome is

• A. Aryl sulphorylase
• B. Trypsin
• C. Acid phosphatase
• D. Cytochromeoxidase

Hint:

Organellar Marker Enzymes:
Lysosome $\rightarrow$ Acid Phosphatase.
Mitochondria $\rightarrow$ Cytochrome Oxidase.
ER $\rightarrow$ Glucose-6-Phosphatase.
Peroxisome $\rightarrow$ Catalase.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept:
Subcellular organelle fractionation employs specific organellar marker enzymes to verify purity and integrity.

Step 2: Detailed Explanation:
Standard subcellular diagnostic marker enzymes:
1. Lysosome: Acid Phosphatase (orthophosphoric-monoester phosphohydrolase, active at $pH \approx 4.5-5.0$).
2. Mitochondria (Inner Membrane): Cytochrome c Oxidase Succinate Dehydrogenase.
3. Endoplasmic Reticulum: Glucose-6-Phosphatase.
4. Peroxisome: Catalase Urate Oxidase.
5. Golgi Apparatus: Galactosyltransferase.
6. Plasma Membrane: $5'$-Nucleotidase $\text{Na}^+/\text{K}^+$-ATPase.

Step 3: Final Answer:
Therefore, the marker enzyme of lysosome is Acid phosphatase, matching option (C).