Question

Larger DNA molecule CANNOT be inserted in host bacterial cell by using ______________.

• A. lambda phage
• B. M13 phage
• C. cosmid
• D. plasmid

Hint:

Cosmids combine features of plasmids and bacteriophages and can carry large DNA inserts.

Answer 1

The Correct Option is B

Step 1: Understanding cloning vectors.
Cloning vectors are DNA molecules used to carry foreign DNA into a host cell for replication.
Step 2: Capacity of different vectors.
Lambda phage and cosmids can carry large DNA fragments.
Plasmids can carry moderately sized DNA inserts.
M13 phage has very limited carrying capacity.
Step 3: Elimination of incorrect options.
Since M13 phage can insert only small DNA fragments, it is unsuitable for large DNA molecules.
Step 4: Conclusion.
Larger DNA molecules cannot be inserted using M13 phage.