Question

In tissue culture technique, to maintain aseptic conditions the nutrient medium is autoclaved at constant pressure of _________ \(\mathrm{lb} / \mathrm{sq}\) inch for continuous __________ minutes.

• A. 10,15
• B. 15,15
• C. 15,20
• D. 20,20

Hint:

Standard sterilisation of plant tissue culture medium uses 15 psi at 121 degrees Celsius.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept
Tissue culture needs aseptic conditions, so the nutrient medium is sterilised in an autoclave.

Step 2: Recall the values
The medium is autoclaved at 15 lb per square inch pressure (about 121\(^{\circ}\)C) for 15 minutes.

Step 3: Conclusion
The values are 15 and 15.

Final Answer:
The medium is autoclaved at 15 lb/sq inch for 15 minutes. \[ \boxed{\text{(B) }\text{15, 15}} \]