Question:
In hybridoma technology, which cells are fused with myeloma cells
In hybridoma technology, which cells are fused with myeloma cells
Show Hint
The spleen is a secondary lymphoid organ packed with mature B-cells, making it the perfect source for antibody-producing cells in immunology.
Updated On: Jul 9, 2026
- Kidney cells
- Liver cells
- Intestinal cells
- Spleen cells
Show Solution
Verified By Collegedunia
The Correct Option is D
Solution and Explanation
Step 1: Understanding the Concept:
Hybridoma technology is a bioengineering method used to produce large quantities of identical antibodies, known as monoclonal antibodies (mAbs).
Step 2: Detailed Explanation:
Monoclonal antibodies are produced by fusing a specific antibody-producing cell with a cancerous cell line that can replicate indefinitely.
Typically, an animal (like a mouse) is immunized with a target antigen.
The animal's spleen is later harvested because it is rich in antibody-producing B-lymphocytes (splenocytes).
These spleen cells are then fused with immortal myeloma cells (cancerous B-cells) using polyethylene glycol (PEG) to create hybridomas.
The resulting hybridoma cells possess both the antibody-secreting capability of the spleen cell and the immortal growth characteristics of the myeloma cell.
Step 3: Final Answer:
Spleen cells (antibody-producing B-cells) are fused with myeloma cells in hybridoma technology.
Hybridoma technology is a bioengineering method used to produce large quantities of identical antibodies, known as monoclonal antibodies (mAbs).
Step 2: Detailed Explanation:
Monoclonal antibodies are produced by fusing a specific antibody-producing cell with a cancerous cell line that can replicate indefinitely.
Typically, an animal (like a mouse) is immunized with a target antigen.
The animal's spleen is later harvested because it is rich in antibody-producing B-lymphocytes (splenocytes).
These spleen cells are then fused with immortal myeloma cells (cancerous B-cells) using polyethylene glycol (PEG) to create hybridomas.
The resulting hybridoma cells possess both the antibody-secreting capability of the spleen cell and the immortal growth characteristics of the myeloma cell.
Step 3: Final Answer:
Spleen cells (antibody-producing B-cells) are fused with myeloma cells in hybridoma technology.
Was this answer helpful?
0
0
Top ICAR AIEEA Fisheries Science Questions
- How does climate variability influence biological productivity and fisheries sustainability?
- The density of seawater at $4^{\circ}\text{C}$ is}
- How does phytoplankton biomass impact fisheries productivity?
- What role do bio-indicators play in assessing aquatic pollution levels?
- How does water pH influence fish physiology and ecology?
View More Questions
Top ICAR AIEEA Economics Questions
- Co-management of natural fisheries resources involves
- GDI measures gender inequalities in achievement in basic dimensions of human development which are
(A). Health
(B). Education
(C). Gender
(D). Economic Status - Borich Training Need Assessment Model discusses
(A). Perceived level of extension
(B). Perceived level of competence
(C). Mean Weighted Discrepancy Scores
(D). Perceived level of importance
Choose the correct answer from the options given below: - Profit maximization of a firm is achieved when:
- The Law of Supply states that, other things remaining constant:
View More Questions
Top ICAR AIEEA Questions
- Which of the following statements is false?
- Which of the following is an example of an inventory?
- According to Adam Smith, productivity gains could be brought about through .
- The supply curve for an artificially scarce good is .
- Which of the following would not be considered an opportunity cost of paid employment?
View More Questions