Question

Given below are two statements.
Statement I - There is no need of agitation in callus culture however it is necessary in suspension culture.
Statement II - During tissue culture techniques, the temperature of the lab is maintained between 18 to 20°C.
In the light of above statements, select the correct option given below:

• A. Both statement I and statement II are correct.
• B. Both statement I and statement II are incorrect.
• C. Statement I is correct but statement II is incorrect.
• D. Statement I is incorrect but statement II is correct.

Hint:

Suspension culture needs agitation, but cultures are kept at about 25 degrees Celsius, not 18 to 20.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept
Callus grows on solid agar medium whereas suspension culture grows in liquid medium.

Step 2: Statement I
Callus needs no shaking, but suspension culture must be agitated for aeration and to prevent clumping.
So Statement I is correct.

Step 3: Statement II
Plant tissue cultures are incubated at about \(25 \pm 2\) degrees Celsius.
The range 18 to 20 is wrong, so Statement II is incorrect.

Final Answer:
Statement I is correct and Statement II is incorrect. \[ \boxed{\text{(C) }\text{Statement I is correct but statement II is incorrect.}} \]