Question:
Given below are two statements:
Statement I : Cryoprotectants are the compounds used in cryoconservation techniques that reduce the freezing point and supercooling point of water
Statement II : Vitrification, encapsulation vitrification and droplet vitrification techniques make use of cryoprotectants
In light of the above statements, choose the most appropriate answer from the options given below:
Given below are two statements:
Statement I : Cryoprotectants are the compounds used in cryoconservation techniques that reduce the freezing point and supercooling point of water
Statement II : Vitrification, encapsulation vitrification and droplet vitrification techniques make use of cryoprotectants
In light of the above statements, choose the most appropriate answer from the options given below:
Statement I : Cryoprotectants are the compounds used in cryoconservation techniques that reduce the freezing point and supercooling point of water
Statement II : Vitrification, encapsulation vitrification and droplet vitrification techniques make use of cryoprotectants
In light of the above statements, choose the most appropriate answer from the options given below:
Show Hint
Cryoprotectants (DMSO, glycerol, PVS2) depress freezing point and prevent ice crystallization, enabling successful vitrification.
Updated On: Sep 15, 2026
- Both Statement I and Statement II are true
- Both Statement I and Statement II are false
- Statement I is correct but Statement II is false
- Statement I is incorrect but Statement II is true
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Verified By Collegedunia
The Correct Option is A
Solution and Explanation
Step 1: Understanding the Concept:
Cryopreservation conserves plant genetic resources at ultra-low liquid nitrogen temperatures ($-196^\circ\text{C}$).
Step 2: Detailed Explanation:
1. Statement I is TRUE: Cryoprotectants (e.g., DMSO, glycerol, ethylene glycol, sucrose) are cell-penetrating or non-penetrating chemical solutes that lower the freezing point, depress the supercooling limit of intracellular water, and increase viscosity to prevent lethal intracellular ice crystal crystallization.
2. Statement II is TRUE: Modern ice-free cryopreservation protocols—including vitrification (PVS2/PVS3 solutions), encapsulation-vitrification (alginate beads), and droplet-vitrification—depend on concentrated cryoprotectant mixtures to induce a meta-stable amorphous glassy state (vitrification) upon rapid plunging into liquid nitrogen.
Step 3: Final Answer:
Therefore, both Statement I and Statement II are true.
Cryopreservation conserves plant genetic resources at ultra-low liquid nitrogen temperatures ($-196^\circ\text{C}$).
Step 2: Detailed Explanation:
1. Statement I is TRUE: Cryoprotectants (e.g., DMSO, glycerol, ethylene glycol, sucrose) are cell-penetrating or non-penetrating chemical solutes that lower the freezing point, depress the supercooling limit of intracellular water, and increase viscosity to prevent lethal intracellular ice crystal crystallization.
2. Statement II is TRUE: Modern ice-free cryopreservation protocols—including vitrification (PVS2/PVS3 solutions), encapsulation-vitrification (alginate beads), and droplet-vitrification—depend on concentrated cryoprotectant mixtures to induce a meta-stable amorphous glassy state (vitrification) upon rapid plunging into liquid nitrogen.
Step 3: Final Answer:
Therefore, both Statement I and Statement II are true.
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