Question

Given below are two statements:
Assertion (A): During fixation, the microorganisms are killed and attached firmly to the microscope slide.
Reason (R): A thin film of material containing microorganisms adequately preserves overall morphology including structures within the cells.

• A. Both (A) and (R) are correct and (R) is the correct explanation of (A)
• B. Both (A) and (R) are correct but (R) is not the correct explanation of (A)
• C. (A) is correct but (R) is not correct
• D. (A) is not correct but (R) is correct

Hint:

Fixation kills microorganisms and attaches them firmly to the slide before staining.

Answer 1

The Correct Option is B

Concept:
Fixation is an important step in microscopic staining of microorganisms.

Step 1: Check Assertion.
During fixation, microorganisms are killed and fixed firmly on the slide. \[ A \text{ is correct} \]

Step 2: Check Reason.
A thin smear helps in proper observation and preservation of morphology during staining. \[ R \text{ is correct} \]

Step 3: Check explanation.
Reason describes the importance of making a thin film or smear, but it does not directly explain the fixation process of killing and attaching microorganisms to the slide. \[ R \text{ is not the correct explanation of } A \] \[ \therefore \text{Correct Answer is (B)} \]