Step 1: Understanding the Concept:
The Weende System of Proximate Analysis is used to chemically analyze feed components.
It partitions animal feed into six fractions: Moisture, Crude Protein (CP), Ether Extract (EE/Crude Fat), Crude Fiber (CF), Nitrogen-Free Extract (NFE), and Ash.
Step 2: Detailed Explanation:
Let us evaluate each statement.
- Statement (A) states that crude fiber represents soluble sugars.
This is incorrect. Crude fiber (CF) represents the insoluble, structural carbohydrates (primarily cellulose, hemicellulose, and lignin) that are resistant to dilute acid and alkali digestion.
Soluble sugars are represented by the Nitrogen-Free Extract (NFE) fraction.
- Statement (B) states that organic matter excludes ash content.
This is correct. Ash represents the total inorganic mineral residue left after burning a feed sample.
Organic matter (OM) represents the combustible portion of the dry matter:
\[ \text{Organic Matter} = \text{Dry Matter} - \text{Ash} \]
Thus, OM excludes ash.
- Statement (C) states that Nitrogen-Free Extract represents the carbohydrate fraction of feed.
This is correct. NFE represents the soluble carbohydrate fraction (such as sugars and starches) and is calculated by difference.
- Statement (D) states that ether extract represents crude fat content.
This is correct. Ether extract (EE) is determined by dissolving the fat-soluble portion of the feed in diethyl ether.
Therefore, statements (B), (C), and (D) are correct.
Step 3: Final Answer:
The correct option is 2, which includes (B), (C), and (D) only.