Question

Find out the incorrect statements regarding Enzymes

• A. Km: Approximate inverse measure of the affinity of the enzyme for a given substrate.
• B. Km: Approximate measure of the concentration of substrate at which the reaction rate is half of the maximum rate.
• C. Enzymes generally function in a broad range of temperature and pH.
• D. 2.7.1.2 is enzyme code for Glucose-6-Phosphotransferase.

Hint:

Km is not just the concentration of substrate at half-maximal reaction rate but also reflects the enzyme's affinity for the substrate. Lower Km values indicate higher affinity.

Answer 1

The Correct Option is B

Analysis of Statements:

✅ Option 1: "Km: Approximate inverse measure of the affinity of the enzyme for a given substrate." 🔹 Correct. Km (Michaelis constant) is an approximate inverse measure of enzyme-substrate affinity. A lower Km indicates higher affinity.

❌ Option 2: "Km: Approximate measure of the concentration of substrate at which the reaction rate is half of the maximum rate." 🔹 Incorrect. Km represents the substrate concentration at which the reaction rate reaches half of Vmax, but it is not just an "approximate measure" of concentration needed for half-maximal activity.

✅ Option 3: "Enzymes generally function in a broad range of temperature and pH." 🔹 Correct. Enzymes have optimal temperature and pH but can still function in a broader range, albeit with lower efficiency.

✅ Option 4: "2.7.1.2 is enzyme code for Glucose-6-Phosphotransferase." 🔹 Correct. The enzyme commission (EC) number 2.7.1.2 belongs to Glucose-6-Phosphotransferase.

Incorrect Statement: Option (2)