Question:

During glycolysis, ATP generation - II step occurs between which of the two intermediates?

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PEPA is the key intermediate that generates ATP when it is converted to pyruvate during glycolysis.
Updated On: Mar 6, 2026
  • 2-PGA and PEPA
  • PEPA and pyruvate
  • 3-PGA and 2-PGA
  • 1,3-diPGA and 3-PGA
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The Correct Option is B

Solution and Explanation

Step 1: Understanding the glycolysis process.
ATP is generated during glycolysis in various steps. The ATP generation - II step specifically occurs between the intermediates PEPA (Phosphoenolpyruvate) and pyruvate.
Step 2: Analyzing the options.
(A) 2-PGA and PEPA: Incorrect, the ATP generation - II step occurs between PEPA and pyruvate, not 2-PGA and PEPA.
(B) PEPA and pyruvate: Correct, ATP is generated when PEPA is converted to pyruvate in the final step of glycolysis.
(C) 3-PGA and 2-PGA: Incorrect, these are involved in earlier steps of glycolysis, not ATP generation - II.
(D) 1,3-diPGA and 3-PGA: Incorrect, ATP is generated earlier in the pathway when 1,3-diPGA is converted to 3-PGA, but not in ATP generation - II.
Step 3: Conclusion.
The correct answer is (B) PEPA and pyruvate, as this step produces ATP in glycolysis.
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