Question

Drug protein binding can be determined by

• A. Chromatography
• B. Dialysis
• C. Phase separation
• D. Langmuir's method

Hint:

Ultrafiltration and equilibrium dialysis are the two gold-standard techniques for protein binding studies.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
Equilibrium dialysis is the standard laboratory technique to study the extent of drug-protein interaction.

Step 2: Detailed Explanation:
In equilibrium dialysis, a protein solution and the drug are placed on one side of a semi-permeable membrane.
The membrane allows the free drug to pass through but retains the protein and the protein-bound drug.
After reaching equilibrium, the concentration of the drug on both sides is measured, allowing the calculation of the fraction of drug bound to the protein.

Step 3: Final Answer:
Dialysis (specifically equilibrium dialysis) is the most common method to determine drug protein binding.