Question

DNA microarray technique is typically not used for: A. Detection of m-RNA, B. Detection of t-RNA, C. Detection of r-RNA, D. Genotyping, E. Detects variant gene sequence limited to SNP.

• A. B and C only
• B. B and E only
• C. B, C and E only
• D. A and D only

Hint:

DNA microarray is mainly used for gene expression analysis using mRNA, genotyping, and SNP detection. It is not typically used for tRNA and rRNA detection.

Answer 1

The Correct Option is A

Concept:
DNA microarray is a molecular biology technique used to detect the expression of many genes at the same time. It is mainly used for mRNA expression analysis, genotyping, and detection of sequence variations such as SNPs.

Step 1: Understand the main use of DNA microarray.
DNA microarray contains many DNA probes fixed on a solid surface. These probes hybridize with complementary nucleic acid sequences from the sample.

Step 2: Check statement A.
Detection of mRNA is one of the most common applications of microarray. In gene expression studies, mRNA is converted into complementary DNA and then hybridized with probes. \[ A \text{ is used in microarray} \]

Step 3: Check statement B.
tRNA detection is not a typical or common use of DNA microarray. tRNA molecules are small and are not usually studied using standard DNA microarray gene expression platforms. \[ B \text{ is typically not used} \]

Step 4: Check statement C.
rRNA detection is also not a typical application of DNA microarray. rRNA is highly abundant and is usually removed or ignored in gene expression analysis. \[ C \text{ is typically not used} \]

Step 5: Check statement D.
Genotyping is a common application of microarray. Microarrays are used to detect genetic variations among individuals. \[ D \text{ is used in microarray} \]

Step 6: Check statement E.
SNP detection is also an important application of microarray. SNP arrays are widely used for identifying single nucleotide polymorphisms. \[ E \text{ is used in microarray} \] Hence, DNA microarray is typically not used for: \[ B \text{ and } C \text{ only} \] \[ \therefore \text{Correct Answer is (A)} \]