Question:

Dinitrogenase is a

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The nitrogenase enzyme has two parts:
- Fe-Protein (Dinitrogenase reductase): Homodimer, ~60-64 KDa.
- MoFe-Protein (Dinitrogenase): Heterotetramer, ~240 KDa.
  • 240 KDa heterotetramer enzyme
  • 200 KDa heterotetramer enzyme
  • 150 KDa heterotetramer enzyme
  • 400 KDa heterotetramer enzyme
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The Correct Option is A

Solution and Explanation

Step 1: Understanding the Concept:
Nitrogenase is the enzyme complex responsible for biological nitrogen fixation (converting atmospheric \(N_2\) to ammonia).
The enzyme consists of two main protein components: Fe-protein (dinitrogenase reductase) and MoFe-protein (dinitrogenase).

Step 3: Detailed Explanation:

The MoFe-protein, or dinitrogenase, is the larger of the two components where the actual reduction of nitrogen takes place.
It is structurally characterized as a heterotetramer of type \(\alpha_2\beta_2\).
It contains two \(\alpha\) subunits and two \(\beta\) subunits.
The total molecular mass of this MoFe-protein complex is approximately 220 to 240 KDa.
Hence, dinitrogenase is described as a 240 KDa heterotetramer enzyme.

Step 4: Final Answer:

Dinitrogenase is a 240 KDa heterotetramer enzyme, making option (A) correct.
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