Question:

Choose the incorrect statements: A) Alien DNA can be linked to origin of replication of DNA. B) DNA ligase can act as a restriction enzyme. C) Each restriction endonuclease recognizes a specific palindromic nucleotide sequence in DNA. D) Exonucleases will cut the specific position of DNA.

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Ligase = Glue; Restriction Enzyme = Scissors (Endonuclease); Exonuclease = Trimmer (cuts from ends).
Updated On: Jun 10, 2026
  • A and B
  • B and C
  • A and C
  • B and D
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The Correct Option is D

Solution and Explanation

Step 1: Concept
Tools of recombinant DNA technology.

Step 2: Analysis
* Statement A is correct: Linking alien DNA to the origin of replication (ori) is necessary for replication. * Statement B is incorrect: DNA ligase joins DNA fragments; it does not cut them like a restriction enzyme. * Statement C is correct: Restriction endonucleases recognize specific palindromic sequences. * Statement D is incorrect: Endonucleases cut at specific internal positions, while exonucleases remove nucleotides from the ends of the DNA strand.

Step 3: Conclusion
Statements B and D are the incorrect statements.

Final Answer: (D)
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