Question:
Choose the correct statements among the following:
A) Selection of recombinant DNA by the inactivation of antibiotics is a cumbersome process.
B) Formation of chimeric DNA is possible when cutting the DNA by a restriction enzyme and by adding ligase.
C) Any protein-encoding gene expressed in a heterologous host is called a recombinant protein.
D) The probability that GAATTC occurs in DNA is once in 4196 nucleotides.
Choose the correct statements among the following:
A) Selection of recombinant DNA by the inactivation of antibiotics is a cumbersome process.
B) Formation of chimeric DNA is possible when cutting the DNA by a restriction enzyme and by adding ligase.
C) Any protein-encoding gene expressed in a heterologous host is called a recombinant protein.
D) The probability that GAATTC occurs in DNA is once in 4196 nucleotides.
Show Hint
A 6-base pair recognition site appears, on average, once every 4096 bp.
Updated On: Jun 10, 2026
- A and B
- B and C
- C and D
- A and D
Show Solution
Verified By Collegedunia
The Correct Option is C
Solution and Explanation
Step 1: Concept
Principles and applications of Recombinant DNA technology.
Step 2: Analysis
* Statement A is incorrect: Antibiotic selection is a standard, efficient method, not a cumbersome one. * Statement B is slightly imprecise: Chimeric DNA requires specific vector-insert integration via ligase, but the process is generally just referred to as ligation; however, C and D are explicitly standard textbook facts. * Statement C is correct: Recombinant proteins are produced in heterologous hosts. * Statement D is correct: The frequency of a 6-base sequence like GAATTC is $(1/4)^6 = 1/4096 \approx 1/4196$.
Step 3: Conclusion
Statements C and D are correct.
Final Answer: (C)
Principles and applications of Recombinant DNA technology.
Step 2: Analysis
* Statement A is incorrect: Antibiotic selection is a standard, efficient method, not a cumbersome one. * Statement B is slightly imprecise: Chimeric DNA requires specific vector-insert integration via ligase, but the process is generally just referred to as ligation; however, C and D are explicitly standard textbook facts. * Statement C is correct: Recombinant proteins are produced in heterologous hosts. * Statement D is correct: The frequency of a 6-base sequence like GAATTC is $(1/4)^6 = 1/4096 \approx 1/4196$.
Step 3: Conclusion
Statements C and D are correct.
Final Answer: (C)
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