Question

As in rDNA technology, in DNA fingerprinting, also, all the following techniques are used EXCEPT:

• A. Restriction enzymes.
• B. Cloning vector plasmids.
• C. Gel electrophoresis.
• D. Isolated DNA segments.

Hint:

Think about which step is needed only when a gene must be carried into and multiplied inside a host cell.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept
DNA fingerprinting needs isolated DNA. It is cut with restriction enzymes, separated by gel electrophoresis, hybridised with labelled VNTR probes and seen by autoradiography.

Step 2: Compare with rDNA technology
Restriction enzymes, gel electrophoresis and isolated DNA segments are common to both methods.

Step 3: Odd one out
A plasmid cloning vector is needed only to carry a foreign gene into a host and multiply it. Fingerprinting never does this.

Final Answer:
Cloning vector plasmids are not used in DNA fingerprinting. \[ \boxed{\text{(B) }\text{Cloning vector plasmids.}} \]