Question

Arrange the following steps of the Polymerase Chain Reaction (PCR) in the correct sequence.
• Primer Annealing
• Denaturation
• Extension by DNA Polymerase Choose the correct answer from the options given below:

• A. 1 \(\rightarrow\) 2 \(\rightarrow\) 3
• B. 2 \(\rightarrow\) 1 \(\rightarrow\) 3
• C. 3 \(\rightarrow\) 2 \(\rightarrow\) 1
• D. 2 \(\rightarrow\) 3 \(\rightarrow\) 1

Hint:

PCR Steps: \[ \boxed{\text{D} \rightarrow \text{A} \rightarrow \text{E}} \] \[ \boxed{\text{Denaturation} \rightarrow \text{Annealing} \rightarrow \text{Extension}} \] Remember: \[ \boxed{\text{PCR uses Taq DNA Polymerase}} \]

Answer 1

The Correct Option is B

Step 1: Recall the purpose of PCR. PCR is a technique used to amplify a specific DNA segment and produce millions of copies.

Step 2: Identify the first step. The DNA strands are first separated by heating. \[ \boxed{\text{Denaturation}} \]

Step 3: Identify the second step. Primers bind to complementary DNA sequences. \[ \boxed{\text{Annealing}} \]

Step 4: Identify the final step. Taq DNA polymerase synthesizes new DNA strands. \[ \boxed{\text{Extension}} \] Hence the correct sequence is: \[ \boxed{\text{Denaturation} \rightarrow \text{Annealing} \rightarrow \text{Extension}} \] \[ \boxed{2 \rightarrow 1 \rightarrow 3} \] Therefore, \[ \boxed{\text{(B)}} \] is the correct answer.