Question

Arrange the following steps of somatic hybridisation in a correct sequence.
A. Digestion of cell walls.
B. Isolation of naked protoplasts.
C. Fusion of protoplasts to get hybrid protoplast.
D. Isolation of single cells from two different varieties of plants.
E. Growing of hybrid protoplast to form a new plant. Choose the correct answer from the options given below:

• A. E, A, B, C, D
• B. D, B, A, E, C
• C. E, B, A, D, C
• D. D, A, B, C, E

Hint:

Think of it as: \textbf{Get cells} $\rightarrow$ \textbf{Take off their coats} (walls) $\rightarrow$ \textbf{Merge them} $\rightarrow$ \textbf{Grow the hybrid}.

Answer 1

The Correct Option is D

Somatic hybridisation is the process of fusing protoplasts from two different plant varieties to create a hybrid:
• Cell Selection (D): Start by isolating single cells from the desired varieties.
• Wall Digestion (A): Use enzymes like cellulase and pectinase to digest the plant cell walls.
• Protoplast Isolation (B): This leaves you with "naked" protoplasts (cells without walls).
• Fusion (C): Fuse the protoplasts (often using PEG) to form a hybrid protoplast.
• Regeneration (E): Culture the hybrid protoplast to grow into a whole new plant.