Question

Arrange the following steps of micropropagation in correct sequence:
A. Selection of explant,
B. Rooting,
C. Multiplication,
D. Hardening, E. Establishment in culture medium. Choose the correct answer from the options given below:

• A. A, B, C, D, E
• B. A, E, C, B, D
• C. C, A, B, D, E
• D. E, A, D, C, B

Hint:

Micropropagation sequence is explant selection \(\rightarrow\) culture establishment \(\rightarrow\) multiplication \(\rightarrow\) rooting \(\rightarrow\) hardening.

Answer 1

The Correct Option is B

Concept:
Micropropagation is the rapid multiplication of plants under tissue culture conditions.

Step 1: Selection of explant.
First, a suitable explant is selected from the mother plant. \[ A=\text{Selection of explant} \]

Step 2: Establishment in culture medium.
The explant is sterilized and placed on a suitable nutrient medium. \[ E=\text{Establishment in culture medium} \]

Step 3: Multiplication.
The established culture is multiplied to produce many shoots. \[ C=\text{Multiplication} \]

Step 4: Rooting.
The shoots are transferred to rooting medium. \[ B=\text{Rooting} \]

Step 5: Hardening.
The rooted plantlets are gradually acclimatized to external conditions. \[ D=\text{Hardening} \] Therefore, the correct sequence is: \[ A,E,C,B,D \] \[ \therefore \text{Correct Answer is (B)} \]